FISH Identifies Chromosome Differentiation Between Contemporary Genomes of Wild Types and the Ancestral Genome of Unisexual Clones of Dojo Loach, <b><i>Misgurnus anguillicaudatus</i></b>

In dojo loach (&lt;i&gt;Misgurnus anguillicaudatus&lt;/i&gt;), although most wild types are gonochoristic diploids that genetically differentiated into 2 groups, A and B, clonal lineages appear in certain localities. Clonal loaches have been considered to hybrid origins between the groups by a series of genetic studies. this study, using FISH with newly developed probe (ManDra-A), we identified 26 (1 pair metacentric 12 pairs telocentric chromosomes) 50 diploid chromosomes contemporary wild-type group loach. contrast, ManDra-A signals were not detected on derived from ancestral The results clearly showed presence differentiations Two-color B-specific ManDra (renamed ManDra-B) probes reconfirmed origin clones identifying both B metaphases. Our clonally reproducing fish possibility chromosomal differentiation can affect gametogenesis. meiotic spermatocytes sex-reversed clones, ManDra-A, ManDra-B, out bivalents. Thus, further support previous conclusion gametogenesis was assured pairing sister duplicated each chromosome or B. study deepens knowledge about association clonality hybridity unisexual vertebrates.